Bu çalışmada 2012 yılı yaz aylarında Aydın ili ve çevresinde bulunan laktasyon
döneminde olan subklinik mastitisli 100 adet keçinin her birinden örneği alınarak Adnan
Menderes Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı’nda Koagulaz
Negatif Stafilokok yönünden incelenmiştir. Çalışmamızda incelenen 100 örneğin toplam
67’sinde (% 67) Koagulaz Negatif Stafilokok izolasyonu gerçekleştirilmiştir.
Çalışmamızda izole edilen 67 suştan 19 (% 28) adedi S. lentus, 17 (% 26) adedi S.
warneri, 12 (% 18) adedi S. haemolyticus, 8 (% 12) adedi S. xylosus, 4 (% 6) adedi S.
schliferi, 3 (% 4) adedi S. cohnii, 2 (% 3) adedi S. caprae ve 2 (% 3) adedi S. hyicus, olarak
belirlenmiştir.
İzole edilen 67 suştan 52 (% 78) adedi ampisiline, 17 (% 25) adedi oksitetrasikline, 7
(% 10) adedi sefaperazona, 55 (% 82) adedi kloksasiline, 10 (% 15) adedi danofloksasine ve
10 (% 15) adedi de enrofloksasine dirençli olarak tespit edilmiştir. Ayrıca 1 (% 1.5) adet suş
oksitetrasikline, 11 (% 16) adet suş enrofloksasine ve 8 (% 12) adet suş da danofloksasine orta
derecede duyarlı olarak bulunmuştur.
Araştırmamızda izole edilen 67 Koagulaz Negatif Stafilokoklar ile yapılan PCR
çalışması sonucunda mecA pozitif olarak identifiye edilen örnek sayısı toplamda 3 (% 4.5)
adettir. 2 (% 3) adet S. warneri ve 1 (% 1.5) adet S. xylosus suşunda mecA geni
identifikasyonu yapılmıştır.
In this study, a total of 100 sampling was made from 100 goat with subclinical mastitis
in lactation period from Aydin Province region in summer time of year 2012, then samples
were brought to Adnan Menderes University Faculty of Veterinary Medicine Department of
Microbiology. A total of 67 (67 %) Coagulase Negative Staphylococci isolates were obtained
out of 100 samples.
In our study, 19 (28 %) of isolates were found as S. lentus, 17 (26 %) of isolates were
found as S. warneri, 12 (18 %) of isolates were found as S. haemolyticus, 8 (12 %) of isolates
were found as S. xylosus, 4 (6 %) of isolates were found as S. schliferi, 3 (4 %) of isolates
were found as S. cohnii, 2 (3 %) of isolates were found as S. caprae and2 (3 %) of isolates
were found as S. hyicus out of 67 isolates which were detected conventionally.
In our study, 52 (78 %) of isolates were found resistant to Ampicillin, 17 (25 %) of
isolates were found resistant to Cefaperazone, 55 (82 %) of isolates were found resistant to
cloxacillin, 10 (15 %) of isolates were found resistant to danofloxacillin, 10 (15 %) of isolates
were found resistant to enrofloxacillin out of 37 isolates. In addition, 1 (1.5 %) of the isolates
were found intermediate susceptible to oxytetracyclin, 11 (16 %) of the isolates were found
intermediate susceptible to enrofloxacillin and 8 (12 %) of the isolates were found
intermediate susceptible to danofloxacillin.
As the same samples were detected by polymerase chain reaction, out of 67 Coagulase
Negative Staphylococci isolates, mecA gene was detected from 3 (4.5 %) of the isolates. mecA
gene was detected from 2 (3 %) S. warneri isolates and from 1 (1.5 %) S. xylosus isolate.
