Rapd fingerprinting of indigenous lysinibacillus fusiformis isolates from stabilized sludge and oil-polluted soil

Abstract

The Lysinibacillus group are motile Gram-positive bacilli, with oval or spherical spores, oxidase and catalase positive, not grouped in strict chains, with strictly oxidative metabolism and very similar to Bacillus sphericus group. Two indigenous isolates of Lysinibacillus fusiformis from oil-polluted soil near gas station and stabilized sludge from petrochemical plant in Serbia (FOV – HIP “Petrohemija”, Pancevo) were tested on IAR and HMT and showed high sensitivity to neomycin, cephalexin and bacitracin, and were resistant to trimethoprim. Both isolates were tolerant to 100μg/ml of Zn and Co, 10μg/ml of Mo, but they differed in tolerance to 20μg/ml of Cd and 10μg/ml of Hg. The isolate from stabilized sludge showed moderate emulsification ability of xylol (E24 65.8) and mineral oil (E24 75.6). The isolate from oil-polluted soil showed very strong emulsification ability of xylol (E24 87.2) and moderate of mineral oil (E24 72.7). RAPD fingerprinting showed clear differences between the two Lysinibacillus fusiformis isolates.