Abstract:
The Lysinibacillus group are motile Gram-positive bacilli, with oval or spherical spores, oxidase and catalase
positive, not grouped in strict chains, with strictly oxidative metabolism and very similar to Bacillus sphericus
group.
Two indigenous isolates of Lysinibacillus fusiformis from oil-polluted soil near gas station and stabilized sludge
from petrochemical plant in Serbia (FOV – HIP “Petrohemija”, Pancevo) were tested on IAR and HMT and
showed high sensitivity to neomycin, cephalexin and bacitracin, and were resistant to trimethoprim. Both isolates
were tolerant to 100μg/ml of Zn and Co, 10μg/ml of Mo, but they differed in tolerance to 20μg/ml of Cd and
10μg/ml of Hg. The isolate from stabilized sludge showed moderate emulsification ability of xylol (E24 65.8) and
mineral oil (E24 75.6). The isolate from oil-polluted soil showed very strong emulsification ability of xylol (E24
87.2) and moderate of mineral oil (E24 72.7). RAPD fingerprinting showed clear differences between the two
Lysinibacillus fusiformis isolates.
