Benign ve malign neoplastik deri lezyonlarında epidermal Langerhans hücreleri ve dermal mast hücreleri

Abstract

Langerhans cells and mast cells are bone marrow derived cells. They represent a critical role in the immune system in the epidermis and dermis. In this present study, we aimed to evaluate the role of mast cells and Langerhans cells in benign and malignant squamous tumors. This study included 12 patients with seborrheic keratosis, 9 patients with actinic keratosis, 15 patients with keratoacanthoma and 33 patients with squamous cell carcinoma. Dermal mast cells and epidermal peri and intratumoral Langerhans cells were labeled with mast cell tryptase and CD1a antibodies respectively. The number of mast cells were significantly increased in the patients with squamous cell carcinoma in comparison with seborrheic keratosis (p=0.001). However, this parameter did not show significant difference between patients of other study groups. The number of intratumoral Langerhans cells in seborrheic keratosis were significantly increased in comparison to squamous cell carcinoma (p=0.026). The peritumoral Langerhans cells were increased in squamous cell carcinoma (p=0.007, p=0.001) and keratoacanthoma (p=0.001, p=0.001) in comparison with actinic keratosis and seborrheic keratosis, respectively. This immunohistochemical study demonstrates that peritumoral Langerhans cells and mast cells are increased in number in keratoacanthoma and squamous cell carcinoma. Epidermal Langerhans cells and dermal mast cells which have roles in the regulation of immune system might have different functions in benign and malignant skin tumors.

Langerhans hücreleri ve mast hücreleri kemik iligi kökenli hücreler olup dermis ve epidermisin immun sisteminde kritik rol oynarlar. Biz bu çalısmada benign ve malign skuamoid tümörlerde mast hücre ve Langerhans hücrelerinin rolünü degerlendirmeyi amaçladık. Bu arastırmaya 12 seboreik keratoz, 9 aktinik keratoz, 15 keratoakantom ve 33 skuamöz hücreli karsinom olgusu alındı. Dermal mast hücreleri ve epidermal peri ve intratümöral Langerhans hücreleri sırasıyla mast hücre triptaz ve CD1a antikorlarıyla boyandı. Mast hücre sayısı skuamöz hücreli karsinomda seboreik keratoza göre anlamlı olarak yüksekti (p=0.001). Mast hücre sayısı diger tümör grupları arasında farklılık göstermedi. Intratümöral Langerhans hücreleri seboreik keratozda skuamöz hücreli karsinoma göre önemli oranda artmıs saptandı (p=0.026). Peritümöral Langerhans hücreleri ise skuamöz hücreli karsinom (p=0.007, p=0.001) ve keratoakantomda (p=0.001, p=0.001), aktinik keratoz ve seboreik keratoza oranla artmıs olarak saptandı. Sonuç olarak biz çalısmamızda skuamöz hücreli karsinom ve keratoakantomda peritümöral Langerhans hücreleri ve mast hücrelerini sayıca artmıs bulduk. Immün sistemde görevleri bulunan epidermal Langerhans hücreleri ve dermal mast hücrelerinin benign ve malign deri tümörlerinde farklı rolleri oldugunu düsündürmektedir.