Estimation of genetic differences among guppies, poecilia reticulata by rapd markers

Abstract

Sixty-four individuals that comprise 8 guppy varieties were used for genetic analysis. Genomic DNA of each individual was extracted from muscle tissues. DNA structure of each sample was examined by using random amplification of polymorphic DNA technique. Nine primers were worked in RAPD-polymerase chain reaction. Seven of them worked well having bands. Genetic similarities and differences for intraspecies and interspecies were calculated. Genetic difference between king and flamingo was the highest (0.604). Genetic similarities of all varieties were determined quietly high (minimum 0.670, maximum 0.948).

Sekiz lepistes varyetesi içeren 64 birey genetik analiz için kullanıldı. Genomik DNA kas dokusundan ekstrakte edildi. Her örneğine ait genomik DNA RAPD tekniği kullanılarak incelendi. Dokuz primer RAPD-PCR'da çalıştırıldı. Bu primerlerden sekiz tanesinde band tespit edildi. Varyeteler içi ve varyeteler arası genetik benzerlik ve farklılık hesaplandı. King ve flamingo arasındaki genetik farklılık en yüksek bulundu (0.604). Tüm varyetelerin genetik benzerlikleri oldukça yüksek olarak bulundu (minimum 0.670, maksimum 0.948).